negative staining is also known as
relief staining
background staining
principle : acidic dyes like india ink , nigrosin , eosin are negatively charged and they donot combine with negatively charged bacterial surfaces and cytoplasm. So dye deposit around the bacterium leaving organisms colorless.
relief staining
background staining
principle : acidic dyes like india ink , nigrosin , eosin are negatively charged and they donot combine with negatively charged bacterial surfaces and cytoplasm. So dye deposit around the bacterium leaving organisms colorless.
No comments:
Post a Comment